在浅表扩散型、结节型、肢端型和恶性雀斑样痣型原发性黑素瘤中BRAF外显子15突变的发生率  

Prevalence of exon 15 BRAF mutations in primary melanoma of the superficial spreading, nodular, acral, and lentigo maligna subtypes

作　　者：Lang J. MacKie R.M 罗素菊 

机构地区：[1]Department of Public Health, University of Glasgow, Glasgow G12 8RZ, United Kingdom Prof.

出　　处：《世界核心医学期刊文摘（皮肤病学分册）》2006年第9期11-12,共2页Digest of the World Core Medical JOurnals:Dermatology

摘　　要：DNA was extracted from 52 thick primary melanomas and mutations sought in exon 15 of the BRAF (v-raf murine sarcoma viral oncogene homolog B1) gene using denaturing high performance liquid chromatograph (dHPLC) fragment analysis, sequencing, and allele-specific PCR. Exon 15 BRAF mutations were found in 13 of 52 (25% ) primary melanomas. These comprised five of 17 (29% ) superficial spreading melanomas, three of 11 (27% ) nodular melanomas, two of 13 (15% ) acral lentiginous melanomas, one of one (100% ) mucosal melanoma and two of 10 (20% ) lentigo maligna melanomas. In common with other groups, our findings show a relative concentration of the exon 15 BRAF mutation in superficial spreading and nodular melanomas, but add further evidence that this mutation not necessary for malignant transformation of the melanocyte.DNA was extracted from 52 thick primary melanomas and mutations sought in exon 15 of the BRAE （v-raf murine sarcoma viral oncogene homolog B1 ） gene using denaturing high performance liquid chromatograph （dHPLC） fragment analysis, sequencing, and allele-specific PCR. Exon 15 BRAF mutations were found in 13 of 52 （25%） primary melanomas. These comprised five of 17 （29%） superficial spreading melanomas, three of 11 （27%） nodular melanomas, two of 13 （15% ） acral lentiginous melanomas, one of one （100%） mucosal melanoma and two of 10 （20%） lentigo maligna melanomas. In common with other groups, our findings show a relative concentration of the exon 15 BRAF mutation in superficial spreading and nodular melanomas, but add further evidence that this mutation not necessary for malignant transformation of the melanocyte.

关 键 词：原发性黑素瘤 恶性雀斑样痣 基因外显子 结节型 突变 扩散 浅表 肢端 

分 类 号：R737.34[医药卫生—肿瘤]