CAT基因敲除对热带假丝酵母产DCA13代谢网络的影响  

Effects of CAT gene knockout on DCA13 metabolic network of Candida tropicalis

作　　者：高弘[1] 黄英明[1] 刘铭[1] 张剑[1] 华玉涛[1] 曹竹安[1] 

机构地区：[1]清华大学化学工程系生物化工研究所,北京100084

出　　处：《化工学报》2006年第9期2157-2161,共5页CIESC Journal

基　　金：国家自然科学基金项目(30000003);国家重点基础研究发展计划项目(2003CB716007).~~

摘　　要：肉毒碱乙酰转移酶在脂肪酸β-氧化过程中起着重要作用.利用同源重组技术,构建了热带假丝酵母CAT基因单拷贝缺失菌CZ-15和双拷贝缺失菌CKC-11后,发现CZ-15的β-氧化过程受到抑制,与原始菌F10-1相比,十三碳二元酸的产量和烷烃转化率都得到了明显提高,菌体生长情况与F10-1基本一致;CKC-11则无法代谢烷烃,菌体生长也受到了较大的影响.采用代谢动力学模型对发酵数据进行了计算与分析,实验结果与模型相符.根据实验和模型计算结果完善了热带假丝酵母代谢葡萄糖和十三碳二元酸的途径.Carnitine acetyltransferase plays an important role in β-oxidation of fatty acid. Candida tropicalis can assimilate n-alkane as a sole carbon source and produce dicarboxylic acids. Starting from a DCA producing strain, F10-1, CAT single and double-knockout strains, CZ-15 and CKC-11 were constructed. The results showed that CZ-15 had the same growth characteristics as F10-1, and due to inhibition of β-oxidation, CZ-15 increased DCA13 yield and molar conversion of alkane; however, CKC-11 could not grow on alkane, and the growth was suppressed. By the established metabolic flux model, the data were calculated and analyzed. Based on the results, more details of the metabolic pathway of glucose and fatty acid in Candida tropicalis were given.

关 键 词：十三碳二元酸 热带假丝酵母 代谢网络 重组菌 

分 类 号：TQ921.7[轻工技术与工程—发酵工程]