产生淀粉糖化酶菌株的诱变选育  

Mutating of Raw Starch-digesting Glucoamylase Strain

作　　者：潘明[1] 周永进[1] 张强[1] 

机构地区：[1]四川理工学院生物工程系,四川自贡643000

出　　处：《酿酒科技》2006年第10期37-39,共3页Liquor-Making Science & Technology

摘　　要：以黑曲霉vin-1为研究对象,通过紫外诱变(UV)和硫酸二乙酯(DES)两次诱变,获得产酶较高的菌株vin-1-24-72,酶活达到527u/g,比原始菌株提高154%,RDA%值为40%。菌株经过7次传代,酶活较稳定。A mutant strain vin-1-24-72 with high yied of raw starch-digesting glucoamylase was isolated from protoplast of Aspergillus niger vin-1 treated by UV and DES. The raw starch-digesting glucoamylase activity of the strain raised to 527 u/g, which was 154 % higher than the enzyme activity ofprotoplast strain. The RDA were 40 % .The enzyme activity of the mutant strain was very steady cultured by seven times.

关 键 词：微生物 黑曲霉 突变 曲利苯兰 硫酸二乙酯 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]