微量血中TC、TG、HDL-C的定量分析  

Quantitative Detection of TC,TG and HDL-C in Micro Level of Blood

作　　者：文镜[1] 张静[1] 王燕伟[1] 桑婷婷[1] 蒋艾[1] 

机构地区：[1]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100083

出　　处：《食品科学》2006年第10期437-440,共4页Food Science

基　　金：北京市教委科技发展计划研究课题(Km200611417003)

摘　　要：为能够用小鼠做实验材料进行降血脂保健食品的动物实验研究,建立了用微量血测定血液中TC、TG和HDL-C的方法。将常规试剂盒检测血液中TC、TG和HDL-C的方法加以改造,使检测TC、TG的采血量分别为30μl,检测HDL-C的采血量为50μl。每只小鼠眼眶采血110μl,离心分离血清后,在原试剂盒方法工作液用量减半的反应体系中进行酶促、显色反应,之后反应液装入600μl比色杯中进行比色测定,即可完成血液中TC、TG和HDL-C的定量。对微量法的精密度、准确度及实际应用情况进行评价,通过与原试剂盒方法比较,证明了微量血试剂盒检测方法的可行性。Establish a quantitative detection method in micro level of blood on the base of reagent box for detecting the TC, TG, and HDL-C in ordinary amount of blood. Methods： For detection of TC, TG, and HDL-C using two parts of 50μl blood. Each mouse only 110 μ1 of orbit blood can be collected. Using half stuff of the reagent box for serum enzyme reaction and color development, than the reaction liquid is poured into a 600μ1 cell for TC, TG and HDL-C colorimetric analysis. Results and conclusion： Mice feed on high lipids food for 30 days a hyperlipemia model can be established. By comparison of the preciseness, accuracy and experimental condition with the primitive reagent box detection, it is revealed that the micro amount blood analysis method is reliable.

关 键 词：检测方法 血清总胆固醇 甘油三酯 高密度脂蛋白胆固醇 

分 类 号：TS201.2[轻工技术与工程—食品科学]