酶解泥鳅蛋白制备小分子肽的工艺研究  

Study on the Processing of the Extraction of Peptide by Enzymatic Hydrolysis of Loach Protein

作　　者：游丽君[1] 赵谋明[1] 崔春[1] 任娇艳[1] 

机构地区：[1]华南理工大学轻工与食品学院

出　　处：《食品与发酵工业》2006年第9期164-167,共4页Food and Fermentation Industries

基　　金：广州科技攻关项目(No.2004Z2-E0151;2004Z1-E0061)

摘　　要：以泥鳅为原料,酶解泥鳅蛋白制备小分子肽。运用响应面分析法优化泥鳅蛋白酶解的工艺条件,探讨了泥鳅蛋白酶解过程中液料比、酶解温度、酶解时间对多肽得率的影响。结果表明,泥鳅蛋白酶解的工艺条件为:液料比1.3、酶解温度57℃、时间9.42h,肽的得率为36.3%(模型预测值为36.37%)。通过OriginPro7.0软件计算,在显著性水平P>0.05下,预测值与实际值无显著不同,表明运用响应面分析法优化泥鳅蛋白酶解工艺条件是可行的。In this study, the loach protein was taken obtained through enzymatic hydrolysis. Response Surface as substrate and the small molecular weight peptide was Methodology（RSM） was used to gain the optimal parameter of processing and to discuss how the parameters influenced the extraction rate of peptide. The results indicated that the optimal parameter of processing was： solvent to material ratio 1.3 （g/g）, extraction temperature 57（℃ ）, extraction time 9.42（h）, the extraction rate of peptide 36.3 % by experiment（the predicted value was 36.37 % ）. The predicted value did not make any evident difference （P〉0.05） through the software of OriginPro7.0. So using Response Surface Methodology（RSM） to gain the optimal parameter of processing was feasible.

关 键 词：泥鳅蛋白 酶解 响应面设计法 肽得率 

分 类 号：TQ464.7[化学工程—制药化工]