丝瓜布固定化米根霉发酵生产L-乳酸  

L-Lactic Acid Fermentation with Immobilized Rhizopus Oryzae by Vegetable Sponge

作　　者：付珊珊[1] 魏顺安[1] 王珍[2] 王远亮[2] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]重庆大学生物材料与生物工程研究中心,重庆400030

出　　处：《重庆大学学报（自然科学版）》2006年第10期114-117,共4页Journal of Chongqing University

基　　金：国家自然科学基金资助项目(30300084)

摘　　要：L-乳酸因其为生产绿色化学材料聚L-乳酸的原料而倍受重视．文中以米根霉As3．6462为菌种，玉米淀粉为原料，研究了用吸附固定米根霉发酵L等L酸．结果表明，相对聚乙烯醇、棉布，丝瓜布有更好的固定化效果．实验条件下，载体边长在8—10mm，载体用量为15mL／50mL种子培养基，玉米淀粉发酵L-乳酸产量为72—83g/L，连续发酵4批后产量基本稳定．丝瓜布固定化米根霉发酵L看L酸有良好的工业的应用前景．Now as a feedstock for the popular biodegradable industry, the L-lactic acid have became more and more important. L-lactic acid fermentation is studied by using Rhizopus oryzae as strain and cornstarch as carbon source. The results show that compared with PVA and cotton, vegetable sponge had the best immobilizing effect. The productivity of Llactic acid is 72 - 83 g/L when the carrier size was about 8 - 10 mm, the concentration of the cartier is 15 ml vegetable sponge per 50 ml seed medium. The immobilized cells can be steadily used in repeated fermentations for more than 4 times. Immobilized by vegetable sponge will have a well appliance in L-lactic acid industry.

关 键 词：L-乳酸 米根霉 固定化 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]