罗非鱼骨骼肌中胶原蛋白分解酶的提取纯化及活性  

Extraction and Activities of the Collagenolytic Enzyme from Muscle of Fresh Tilapia

作　　者：赵元晖[1] 曾名勇[1] 郭瑶[1] 董士远[1] 刘尊英[1] 

机构地区：[1]中国海洋大学食品工程学院,山东青岛266003

出　　处：《现代食品科技》2006年第2期40-42,共3页Modern Food Science and Technology

摘　　要：新鲜罗非鱼肉匀浆离心后经硫酸铵分级沉淀、SephadexG-100凝胶柱层析、DEAESephadexA-50阴离子交换后,得到经SDS-PAGE证实的纯品。650g罗非鱼骨骼肌可制备总活力14835U,比活力526.4U/mg的胶原蛋白分解酶,纯化倍数为111.53倍。Collagenolytic enzyme was isolated and purified from the skeletal muscles of 650g fresh tilapia using 40%-80% saturation ammonium sulfate fraction, Sephadex G-100 gel filtration and DEAE Sephadex A-50 anion exchange chromatogram column, The result showed 14835U collagenolytic enzyme with specific activity 526.4U/rag was obtained.

关 键 词：罗非鱼 胶原蛋白酶 凝胶层析 阴离子交换 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]