18S rRNA基因siRNA表达质粒的构建和鉴定  

Construction and identification of siRNA expression plasmid for interference buffalo 18S rRNA

作　　者：王维[1] 钱建新[1] 董克家[1] 

机构地区：[1]海南医学院转基因实验室,海南海口571100

出　　处：《海南医学院学报》2006年第5期393-395,共3页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(NO:30360039)

摘　　要：目的:构建和鉴定水牛18SrRNA干扰真核表达载体,为进一步研究18SrRNA功能奠定基础。方法:人工设计合成水牛18SrRNA-sh565干扰序列,经基因重组定向克隆插入到真核表达载体pGPH1/GFP/Neo,随机挑选2个克隆菌提取质粒并进行酶切和测序鉴定。结果:质粒酶切和测序结果显示,siRNA寡核苷酸按正确方向和序列插入质粒。结论:成功构建pGPH1/GFP/Neo-buffalo-18S rRNA-sh565干扰表达质粒。Objective： To construct and identify siRNA expression plasmid for interference buffalo 18S rRNA. Methods： Designed buffalo-18S rRNA-sh565 oligonucletides were chemically synthesized and inserted into pGPH1/GFP/Neo vector after annealing, then transformed into E.Coli DH5α. The recombinant plasmid was identified by restriction endonuclease and DNA sequencing. Results： DNA sequencing showed the siRNA oligonucletides were correctly inserted into the eukaryotic expression vector pGPH1/GFP/Neo. Conclusion： siRNA expression plasmid for interference buffalo 18S rRNA is successfully constructed and lays the foundation for further study of 18S rRNA

关 键 词：RNA干扰 18S RRNA 基因表达 真核细胞 质粒 水牛 

分 类 号：G344.11[文化科学]