破碎酵母释放乙醛脱氢酶的研究  

Study on Aldehyde Dehydrogenase Release by Breaking Yeast Cells

作　　者：吴桂英[1] 吴元欣[1] 赵玉凤[1] 王存文[1] 朱圣东[1] 池汝安[1] 

机构地区：[1]武汉工程大学化工与制药学院,湖北武汉430073

出　　处：《酿酒科技》2006年第11期21-23,共3页Liquor-Making Science & Technology

基　　金：湖北省新世纪高层次人才工程入选人员科研项目择优资助;新世纪百千万人才工程国家级人选科研项目资助;湖北省国际科技合作重点项目计划

摘　　要：乙醛脱氢酶(ALDH)是一种胞内酶,在从酵母中分离提取ALDH的过程中,破碎细胞是其中的关键步骤之一。本文着重考察了自溶、反复冻融、超声波和高压破碎仪的细胞破碎方法对释放ALDH的影响。结果表明,用高压破碎仪破碎,酵母细胞破碎率可达到95%以上,而且有效地防止了ALDH的分解及降解,是一种较为理想的破碎方法。Aldehyde dehydrogenase （ALDH） is a kind of endoenzyme and cell-breaking is one of the key procedures in the extraction of ALDH from S.cerevisiae. The effects of different cell-breaking methods including self-dissolving, repetitive freezing and thawing and ultrasonication and high pressure cracker on ALDH release were studied and compared in this paper. The results showed that cell-breaking by high press cracker was the best methord with its breaking rate above 95 % and it could effectively prevent ALDH decomposition and degradation.

关 键 词：微生物 酵母细胞 乙醛脱氢酶 破碎方法 

分 类 号：TS261.11[轻工技术与工程—发酵工程]