酶性氧化合成茶黄素条件优化的研究  

Study on the Optimum Condition of Synthesizing Theaflavins by Using Enzyme-catalysing Oxidation

作　　者：谷记平[1] 刘仲华[1] 黄建安[1] 施兆鹏[1] 

机构地区：[1]湖南农业大学天然产物工程技术研究中心

出　　处：《茶叶科学》2006年第4期285-290,共6页Journal of Tea Science

基　　金：湖南省科技厅重点项目(03JZY3016);科技部十五攻关项目(2004BA542C)

摘　　要：利用提取粗酶液在单因素试验和正交试验进行合成茶黄素以及在此基础上利用捣碎茶鲜叶(不提取粗酶液)直接进行合成茶黄素试验以确定最优反应条件。结果表明,在本试验反应体系中,茶黄素酶促合成的最佳条件(茶黄素总量)为:温度为30℃,反应体系的最佳pH值为4.8,底物浓度为0.9g/100ml,酶源物浓度为28g/100ml(以鲜叶重量计),通氧量为0.4L/min,最佳反应终止时间是40min。其中温度和pH值是反应体系中两个极其重要的影响因素(P<0.05);通氧量也是影响茶黄素酶性合成的必不可少的条件之一。Obtain the optimum reaction condition of synthesizing theaflavins by enzymatic oxidation, synthesizing theaflavins was studied by raw ppo extracted from fresh tea leaves under single factor and orthogonal experimental design. Based on these results, synthesizing theaflavins was also studied by using breaking fresh tea leaves. The result showed that the optimum condition of theaflavins synthesizing is 30℃ temperature is, under this reaction system, pH value of the present system is 4.8, the concentration of substrates is 0.9 g/100 ml, the concentration of enzymes is 28 g/100 ml （based on the fresh weight）, oxygen rate is 0.4 L/min. Temperatures and pH values in the reaction system are the most important factors（P〈0.05）, and adding suitable oxygen is another necessary factor in theaflavins synthesis by enzymatic oxidation.

关 键 词：茶黄素 儿茶素 酶性氧化 最优条件 

分 类 号：S571.1[农业科学—茶叶生产加工]