L-亮氨酸发酵培养基优化试验  

Study on medium optimization of L-leucine fermentation

作　　者：伍时华[1] 徐雅飞[1] 张健[1] 廖兰[1] 黄翠姬[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006

出　　处：《广西植物》2006年第6期692-696,701,共6页Guihaia

基　　金：广西科学基金项目(GKQ0229012)资助~~

摘　　要：应用Plackett-Burman设计试验从L-亮氨酸基础发酵培养基的10种成分中筛选出5种重要成分,然应用响应面分析试验确定5种重要成分的最适用量。培养基优化后摇瓶分批发酵72h产L-亮氨酸18.Five main factors were screened by the Placket-Burman design method from ten ingredients of L-leucine basic fermentation medium. Then,the best level of five factors was determined by response surface analysis. The strain TQ9806-1 could produce L-leucine 18. 05 g/L after fermentation with optimum medium for 72 hours by flask-shaking batch fermentation.

关 键 词：L-亮氨酸 发酵培养基 优化 响应面分析 

分 类 号：TQ922[轻工技术与工程—发酵工程]