啤酒酵母原生质体融合株HN31-6的啤酒酿造中试研究  

Pilot Beer Brewing Test of Protoplast Fusant HN31-6 of Saccharomyces cerevisiae

作　　者：刘月英[1] 王芬[1] 由媛[1] 蔡车国[1] 戴玉聪 全丽 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]福建雪津啤酒有限公司,福建莆田351111

出　　处：《厦门大学学报（自然科学版）》2006年第6期855-858,共4页Journal of Xiamen University：Natural Science

基　　金：福建雪津啤酒有限公司资助

摘　　要：为了考察啤酒酵母融合株HN31-6的啤酒酿造生产性能,以12.5°Bx麦芽汁为培养基,用500 L发酵罐在12℃下发酵,发酵期间每天取样测定发酵液的发酵度和酒精度以及发酵液的双乙酰、乙醛、高级醇和酯类等挥发性物质的含量.结果表明:在上述条件下,发酵14 d,发酵液的发酵度和酒精度分别为69.9%和4.46%;双乙酰和乙醛的含量分别为0.036 4 mg/L和4.53 mg/L;异戊醇、异丁醇和正丙醇的含量分别为46.47、8.34和9.82 mg/L,总高级醇的含量为64.6mg/L;甲酸乙酯、乙酸乙酯、乙酸异丁酯、乙酸异戊酯和己酸乙酯的含量分别为0.132、21.92、0.037、2.22和0.293 mg/L,总酯为24.6 mg/L;该菌株的发酵度以及发酵液的酒精、双乙酰、乙醛、总高级醇和一些酯类等的含量均达到国家优质啤酒的标准,啤酒口感良好.该融合株在啤酒酿造生产中具有很好的应用前景.In order to inspect the characters of beer brewing of protoplast fusant HN31-6 of Saccharomyces cerevisiae,the fermentation test was carried out under the conditions of 500 L fermentor with 400 L 12.5° Bx wort as the medium at 12℃ for 14 days. The fermentative degree of the fermented liquid and the contents of diacetyl etc volatility substances were analyzed during the fermentation. The results showed that the fermentative degree and alcohol degree of the fermented liquid of fusant HN31-6 under the abovementioned conditions were 69.9% and 4.46%, respectively. The contents of diacetyl and acetaldehyde in the fermented liquid were 0. 036 4 mg/L and 4.53 mg/L, respectively. The contents of isopentitol, isobutylalcohol and n-propyl- alcohol were 46.47,8.34 and 9.82 mg/L, respectively,and the content of total higher alcohols was 64.6 mg/L in the fermented liquid. The contents of methylacerate, ethyl acetate, iaobutylacetate, isoamylacetate and ethylcaproate were 0. 132,21.92,0. 037,2, 22 and 0.293 mg/L, respectively and total ester was 24.6 mg/L. The fermentative degree of the fermented liquid and contents of diacetyl,acetaldehyde and total higher alcohols and some esters in the fermented liquid all achieved the standards of high quality beer. The sensory evaluation of the beer produced by fusant HN31-6 was nice. The fusant HN31-6 has a good prospect in the application for the beer brewing.

关 键 词：啤酒酵母 原生质体融合株 啤酒酿造 中试 

分 类 号：Q503[生物学—生物化学]