烟草中β-胡萝卜素的热裂解产物的研究  

Study of Pyrolysates of β-Carotene in Tobacco

作　　者：杨伟祖[1] 谢刚[1] 王保兴 侯英 杨勇 徐济仓 杨燕 王玉 

机构地区：[1]昆明理工大学,云南昆明650093 [2]云南瑞升科技有限公司,云南昆明650106

出　　处：《色谱》2006年第6期611-614,共4页Chinese Journal of Chromatography

基　　金：国家烟草专卖局基金资助项目(No.110200101044);云南省烟草专卖局基金资助项目(No.2003B203)

摘　　要：为了研究烟草中β-胡萝卜素的高温裂解产物对卷烟抽吸品质的影响,利用热裂解气相色谱/质谱联用仪在不同裂解氛围(空气、氮气中含10%O2及N2)和不同温度(300,600和900℃)下对β-胡萝卜素进行裂解,裂解产物用固相微萃取装置进行吸附,然后将吸附到的裂解产物用气相色谱/质谱联用仪(GC/MS)进行分析。结果表明,β-胡萝卜素在不同裂解条件下主要的裂解产物是甲苯、对二甲苯、1,2,3,4-四氢-1,1,6-三甲基萘和2,7-二甲基萘等化合物,另外还生成异佛尔酮、β-环柠檬醛、β-紫罗兰酮、二氢猕猴桃内酯等香味化合物,这些物质随裂解温度和裂解氛围的不同其含量有所差异。Relationships between tobacco compounds and smoke products are complex and often difficult to unravel. Pyrolysis experiments have frequently been used to establish such relationships. The relevance of pyrolysis experiments to the behavior of tobacco constituent in a burning cigarette was studied. A set of pyrolysis conditions has been developed to study the effect of thermal degradation of β-carotene to the cigarette smoke quality, and the conditions was approximated to those occurring in the pyrolysis region of the burning cigarette. The pyrolysates of β-carotene were investigated in air, 1.0%O2 （in N2） and N2 at three temperature levels of 300 ℃, 600 ℃ and 900 ℃, respectively. The pyrolysates were adsorbed by solid-phase microextraction （SPME） and then analyzed by gas chromatography/mass spectrometry （GC/ MS）. Under the different conditions, the major pyrolysates from r-carotene are hydrocarbon compounds such as toluene, p-xylene, 1,2,3,4-tetrahydro-1, l ,6-trimethyl-naphthalene and 2, 7-dimethyl-naphthalene, and some important flavors existing in cigarette smoke such as isophorone, 2,6,6-trimethyl-1 -cyclohexene-1-carboxaldehyde, β-ionone and 5,6,7,7a-tetrahydro-4,4, 7a-trimethy.l-2 （4H）-benzofuranone. The amount of these pyrolysates alters with the change of pyrolysis temperature levels and the concentration of oxygen.

关 键 词：气相色谱/质谱法 固相微萃取 Β-胡萝卜素 烟草 热裂解 

分 类 号：O658[理学—分析化学]