不同酶切方式制备的壳寡糖抑菌活性的研究  

Antimicrobial activity of chitooligosaccharides prepared by different enzymolysis manners

作　　者：陈建国[1] 郑连英[1] 

机构地区：[1]浙江大学生物工程研究所,浙江杭州310027

出　　处：《浙江大学学报（工学版）》2006年第11期1905-1908,共4页Journal of Zhejiang University：Engineering Science

基　　金：浙江省重点科技项目(2005C-22001);国家自然科学重点基金资助项目(50233020)

摘　　要：壳寡糖可以抑制多种病原微生物的生长,其抑菌活性与其数均相对分子质量有密切关系.由专一性的壳聚糖混合酶、内切酶和外切酶降解制备3种不同数均相对分子质量的壳寡糖C-1、C-2和C-3样品,通过抑制生长速率的方法,研究了3种壳寡糖对几种常见植物病原菌的拮抗作用,并采用抑菌圈法测定了壳寡糖对植物病原菌的最低抑菌质量浓度.结果表明,C-1和C-2都有较好的抑菌活性,C-2的抑菌活性明显优于C-1,C-3基本上没有抑菌活性,反而促进了植物病原菌的生长.抑菌效果因壳聚糖酶的酶切方式、壳寡糖的质量浓度和供试菌种而异.Chitooligosaeeharides ean inhibit many mieroorganisms, with its antimierobial aetivity being related to its molecular weight. The chitooligosaceharides C-1, C-2 and C-3 were prepared respectively by chitosanase, endo-chitosanase and exo-chitosanase. The antimicrobial activity of three chitooligosaccharides with different molecular weights were examined against seven pohytopathogenic fungi by determining the inhibitory growth curves. The minimum inhibitory concentration of ehitooligosaeeharides C-1 and C-2 were examined by determining inhibitory circles. C-1 showed higher antimicrobial activity than C-2, C-3 had no antimicrobial activity nearly, and to some extent, C-3 promoted the growth of some pohytopathogenie fungi. The effect of antimierobial activity depends on species of enzymolysis, concentration of ehitooligosaecharide and categories of pohytopathogenie fungi.

关 键 词：壳寡糖 壳聚糖酶 壳聚糖内切酶 抑菌活性 

分 类 号：TQ050.425[化学工程]