双水相提取糖化酶的研究  

Study on the extraction of glucoamylase by double aqueous phase system

作　　者：杜学敏[1] 武金霞[1] 张贺迎[1] 李红丹[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《中国酿造》2007年第1期7-9,49,共4页China Brewing

基　　金：河北省生物工程重点学科资助

摘　　要：利用PEG/(NH4)2SO4双水相系统从粗酶液中提取糖化酶。研究了PEG、(NH42)SO4、NaCl溶液浓度对糖化酶分配系数、回收率以及分相时间的影响,确定了提取糖化酶的最佳条件,即PEG溶液浓度为24%,(NH42)SO4溶液浓度为20%时,分配系数最小为0.0120,糖化酶回收率最高为98.81%。The glucoamylase was extracted and purified by PEG/（NH4） 2SO4 double aqueous phase system. The effects of the concentration of PEG, （NH4）2SO4 and NaCI solution on distribution coefficient, recycling rate and phase separation time were studied. The optional conditions for glucoamylase extraction were determined. When the concentration of PEG solution was 24% and that of （NH4）2SO4 solution was 20%, the smallest distribition coefficient and highest recycling rate were obtained as 0.0120 and 98.81%, respectively.

关 键 词：糖化酶 PEG/(NH4)2SO4 双水相 

分 类 号：Q814.1[生物学—生物工程]