盐酸克仑特罗ELISA试剂盒的研制  

Development of Enzyme Immunoassay Kit for Clenbuterol

作　　者：李鹏[1] 赖卫华[1] 陈雪岚 陈媛 金晶[1] 田瑞丽[3] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047 [2]江西中德生物工程有限公司,江西南昌330029 [3]河北科技大学化工与环工学院,河北石家庄050018

出　　处：《食品科学》2006年第12期296-300,共5页Food Science

基　　金：科技部2005年度中小企业创新基金项目(05C26213600918)

摘　　要：在间接竞争酶联免疫吸附法的基础上,研制了一种可以检测尿样或食品中盐酸克仑特罗的试剂盒。ELISA检测方法的各个影响因素均通过实验进行了优化。该试剂盒具有良好的灵敏度,半抑制率(IC50)为0.6ng/ml,该抗体与肾上腺素等结构类似物的交叉反应率均小于0.3%,回收率为90%～104%,批内变异系数小于6%,批间变异系数小于10%,试剂盒在12个月保存期内稳定。可用于盐酸克仑特罗的快速定量检测。An indirect competitive enzyme immunoassay with monoclonal antibody was established for measuring clenbuterol in urine or food. The effect factors of ELISA was optimized by experiment. The kit was showed good sensitivity with the IC50 of 0.6ng/ml. The kit generally had not more than 0.3% cross-reactivity towards adrenergic agonists. The recoveries of clenbuterol spike in pig urine were 90%～104%. The inter-assay coefficient variation was below 6%. The intra-assay coefficient variation was below 10%.The kit also Showed fine precision and accuracy of assay in 12 months shelf life.

关 键 词：盐酸克仑特罗 酶联免疫吸附法 试剂盒 

分 类 号：TS207.7[轻工技术与工程—食品科学]