高效液相色谱法测定苦瓜啤酒中亮蓝  

Determination of Bright Blue in Bitter Gourd Beer by High Performance Liquid Chromatography

作　　者：龙洲雄[1] 胡海山[1] 万春花[1] 鄢兵[1] 

机构地区：[1]江西省分析测试研究所,江西南昌330029

出　　处：《食品科学》2006年第12期657-659,共3页Food Science

摘　　要：本文采用聚酰胺粉吸附提取苦瓜啤酒中的色素亮蓝。用HPLC法测定,采用KromasilC18柱,乙腈-0.02mol/L乙酸铵溶液为流动相,梯度洗脱,流速1.0ml/min,检测波长627nm。回收率在89.6%～97.6%之间,RSD(n=6)为3.25%,最低检出浓度为0.002mg/kg。实验结果表明该法快速、简便、准确可靠。An HPLC method was developed to determine bright blue in bitter gourd beer. After being extracted with polyamide resin, bright blue could be adsorbed on Kromasil C18 column, and eluted gradiently by using acetonitrile -0.02mol/L ammonium acetate as the mobile phase at flow rate 1.0ml/min, and be detected at 627nm. The recovery of sample is in the range of 89.6%～97.6%, RSD（nffi6） is 3.25%. This method is proved to be simple, rapid and accurate.

关 键 词：高效液相色谱 苦瓜啤酒 亮蓝 

分 类 号：TS262.5[轻工技术与工程—发酵工程]