海产品中几种危害物质快速检测方法的比较  

Comparison of Rapid Detection of Hazardous Substance in Seafood

作　　者：王亚群[1] 林洪[1] 王静雪[1] 靳晓梅[2] 

机构地区：[1]中国海洋大学水生生物制品安全性实验室,山东青岛266003 [2]青岛市卫生局卫生监督所,山东青岛266000

出　　处：《水产科学》2007年第1期26-30,共5页Fisheries Science

基　　金：科技部食品安全重大专项(2001BA804A51)

摘　　要：为了控制餐饮业中海产品的卫生状况,避免发生中毒事件,保证餐饮业的良好的卫生条件,对海产品中的甲醛、亚硫酸盐、过氧化氢等几种危害物质的快速检测方法进行了比较。通过对几种危害物质快速定性法的比较,筛选出准确性好、灵敏度高、操作简单、检测快速、试剂易保存以及成本低廉的快速检测方法。对甲醛、亚硫酸盐、过氧化氢的检测均可采用相应的试纸法,检出限分别为50μg/m l、50μg/m l、和10μg/m l。甲醛的检测也可以采用间苯三酚法、亚硫酸品红法和FeC l3-盐酸苯肼法,检出限分别为10μg/m l、1μg/m l和10μg/m l。In order to control the sanitation of seafood in the restaurant industry and avoid incident of poisoning, different detecting methods of the hazardous substance in seafood-formaldehyde, sulfites and hydrogen peroxide were studied. In this papcr, both chemical quantitative methods and rapid qualitative methods were used to study on the veracity, the limit of detection, the speed, the complicacy, the cost and the preservation of reagents, then relative ideal methods were chosen. The method of test paper is especially fitted to spot detection. The limit of detection of formaldehyde, sulfites and hydrogen peroxide are 50μg/ml, 50 μg/ml and 10 μg/ml, respectively. Phloroglucinol method, sulfurous acid fuchsine method and FeCl3-Phenylhydrazine hydrochloride method are also recommended for the detection of formaldehyde, with a limit of detection of 10 μg/ml, 1 μg/ml and 10 μg/ml, respectively. However, these methods are not suitable for spot detection since liquid reagent used in these methods is not portable and hard to preserve.

关 键 词：海产品 危害物质 快速检测 甲醛 亚硫酸盐 过氧化氢 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]