Bacillus sp.HA-1菌株的选育及特性研究  

Breeding of CGTase Processing Strain with N+ Ion Implantation

作　　者：王雁萍[1] 陈林海[1] 李宗伟[1] 秦广雍[1] 李宗义[2] 谈重芳[1] 段宇珩[1] 霍裕平[1] 

机构地区：[1]河南省离子束生物工程重点实验室,河南郑州450052 [2]河南师范大学生命科学学院,河南新乡453007

出　　处：《食品科学》2007年第1期161-164,共4页Food Science

基　　金：河南省科技攻关项目(001160201)

摘　　要：应用N+离子注入诱变技术对环糊精葡萄糖基转移酶产生菌进行诱变选育高产菌株。筛选到产酶水平是出发菌株1.6倍的菌株B.sp.HA-1,该菌株于5m3生产罐上发酵24h产酶能力达到6800IU/ml。酶转化25%木薯淀粉生成β-环糊精,12h转化率达到85%。该菌株产酶具有良好的传代稳定性,F2～20代产酶水平是F1代的98.11%～102.66%。With N^＋ ion implantation, a high-yield cyclodextrin glucanotransferase processing strain Bacillus sp.HA-1 was selected, with CGTase activity level 1.6 times of that of original strain. Its CGTase activity reached 6800IU/ml when cultivated for 24 hours in 5m^3 fermentor. Using the CGTase to transform 25% tapioca starch into 13 -CD, the convertion rate in 12 hours is 85%. The CGTase activitise of F2-F20 are ranged from 98.11% to 102.66% as compared with F1, which proves that the genetic quality ofB. sp. HA-1 strain is stable.

关 键 词：N^+离子注入 环糊精葡萄糖基转移酶 Β-环糊精 选育 发酵 

分 类 号：Q933[生物学—微生物学]