榛蘑多糖的分离鉴定及其清除氧自由基作用研究  

Isolation and Identification of Saccharides from Armillaria mellea and Their Effects on Scavenging Oxygen Free Radicals

作　　者：杨立红[1] 黄清荣[1] 冯培勇[1] 姜华[1] 蔡德华[1] 刘静[1] 

机构地区：[1]烟台师范学院生命科学学院,山东烟台264025

出　　处：《食品科学》2007年第1期309-313,共5页Food Science

基　　金：山东省科技厅攻关项目(011100108)

摘　　要：榛蘑烘干后经热水提取,乙醇沉淀得粗多糖。粗多糖经Sevag法除蛋白后上DEAE-纤维素(OH-)柱层析,分离得到五种多糖组分Am-I、Am-II、Am-III、Am-IV和Am-V,SepharoseCL-4B测得Am-I分子量为1.46×104,红外光谱和NMR波普分析表明Am-I为含有葡萄糖醛酸的主要以β(1,3)糖苷键和β(1,6)糖苷键连接的D-吡喃葡聚糖。邻苯三酚自氧化法测定榛蘑粗多糖和Am-I均具有清除氧自由基的作用。The saccharides ofArmUlaria mellea were extracted by hot water and then deposited by ethanol. The DEAE cellulose column chromatography was used on saccharide purification after the protein was extracted with Sevage method. The results indicated that the saccharides are composed of Am-Ⅰ, Am Ⅱ, Am Ⅲ, Am Ⅳand Am Ⅴ. The Sepharose CL-4B method showed that molecular weight of Am-Ⅰis 1.46 ×10^4 when Infrared Ray（IR）and Magnetic Resonance（NMR） assays showed that Am-Ⅰ is glucopyranose（containsing glucuronic acid）, mainly linked by β（1-3）glucosidic linkage and β （1-6） glucosidic linkage. The pyrogallic acid autooxidation method assay indicated both saccharides and Am-Ⅰ have anti-oxidant property.

关 键 词：榛蘑 多糖 分离鉴定 清除氧自由基 

分 类 号：S646.19[农业科学—蔬菜学]