慢性鼻窦炎患者鼻咽部表面活性物质磷脂构成的测定  

Measurement of components of the phospholipid of the surfactant in irrigating fluid from the nasopharynx of patients with chronic sinusitis

作　　者：吉晓滨[1] 王群芳[2] 谢景华[1] 杨凯[2] 

机构地区：[1]广州市第一人民医院耳鼻咽喉科,广州510180 [2]华南师范大学化学与环境学院分析测试中心

出　　处：《临床耳鼻咽喉头颈外科杂志》2007年第2期63-66,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广州市卫生局医药卫生科技项目基金资助(2004年)

摘　　要：目的:探讨慢性鼻窦炎对鼻咽部表面活性物质磷脂的构成是否产生影响,并对磷脂成分进行研究。方法:采集慢性鼻窦炎患者(慢性鼻窦炎组)和健康人(对照组)鼻咽部灌洗液,用高效液相色谱方法对表面活性物质磷脂4种成分即磷脂酰丝氨酸(PS)、磷脂酰乙胺醇(PE)、磷脂酰胆碱(PC)和鞘磷脂(SP)的构成比进行测定。结果:①鼻咽部存在表面活性物质,磷脂4种成分均可测出;②慢性鼻窦炎组与对照组比较,PS的构成比差异有统计学意义(P<0.05),含量最多的主要成分PC差异有统计学意义;③慢性鼻窦炎Ⅲ型与对照组PS比较,构成差异有统计学意义(P<0.05),随鼻窦炎分型分期的进展,其构成比有逐渐降低的趋势。结论:①鼻咽部存在表面活性物质,其磷脂成分有PS、PE、PC和SP,PC构成比最多,对鼻咽部表面活性物质的活性起决定作用;②慢性鼻窦炎可引起鼻咽部表面活性物质磷脂部分成分减少;③随分型分期进展,部分磷脂成分的构成比逐步降低,进一步表明可能引起咽鼓管表面活性物质磷脂成分的改变,引起中耳、咽鼓管功能不全。Objective：To investigate the effect of chronic sinusitis on components of the phospholipid of nasopharyngeal surfactant, and to study biochemical component of phospholipid of surface active substance. Method： The concentrations of surfactant in nasopharyngeal irrigating fluid were implemented in normal controls and patients with chronic sinusitis. Components of phospholipid such as Phosphatidylserine, Phosphatidylethanolamine, Phosphatidylcholine and Sphingophospholipid were measured by the high-performance liquid chromatograph. Resnlt：Results showed as follows （1）There was surfactant in nasopharynx. 4 compositions of phospholipid could be measured. （2）Compered with controls, Phosphatidylserine signifcantly decreased in patients with chronic sinusitis （P 〈0.05）. （3）Only Phosphatidylserine signifcantly decreased between sinusitic 11 stages and controls （P 〈 0, 05）. The rests had no signifcant difference between chronic sinusitis ＂stages and controls, and among stages. But as the chronic sinusitis＂ stages proceeded, proportion of Phosphatidylserine may decreased. Conclusion： （1） There is surfactant in nasopharynx, nasopharyngeal surfactant is made of Phosphatidylserine, Phosphatidylethanolamine, Phosphatidylcholine and Sphingophospholipid. The proportion of Phosphatidylcholine shows most, and determines biochemical effect of nasopharyngeal surfactant. （2）Chronic sinusitis may cause decrease of some com- ponents of nasopharyngeal surfactant. （3）As the chronic sinusitis＂ stages proceed, the proportion of some phospholipids progressively decrease. Which, above assessed, may cause the change of surfactant in eustachian tube, and cause dysfunction of middle ear and eustachian tube.

关 键 词：鼻窦炎 磷脂类 鼻咽 

分 类 号：R739.63[医药卫生—肿瘤]