乳腺癌中p16基因缺失与突变的研究  

Analysis of p16 Gene Deletion and Mutation in Breast Cancer

作　　者：李铁臣[1] 刘平[2] 孙惠兰[1] 徐毓其[1] 

机构地区：[1]皖南医学院遗传医学研究室 [2]皖南医学院附属弋矶山医院肿瘤科,安徽芜湖241001

出　　处：《癌变．畸变．突变》2007年第1期73-75,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:了解乳腺癌与p16基因的关系。材料与方法:采用PCR和DNA测序方法,对33例乳腺癌患者肿瘤组织标本的p16基因进行检测,研究p16基因的缺失和突变情况。结果:所有标本均未检出纯合缺失,10例标本进行了外显子2的序列测定,也未发现点突变。结论:p16基因的纯合缺失和点突变可能与乳腺癌的发生无关。BACKGROUND ＆ AIM： To investigate p16 gene alterations in breast cancer. MATERIALS AND METHODS： 33 fresh tumor specimens taken from breast cancer patients were analyzed for p16 gene deletion and mutation by polymerase chain reaction （PCR） and DNA sequencing, RESULTS： No homozygous deletion of p16 gene exon 1, 2 and 3 was observed in any of the 33 cases and no point mutation of pI6 gene exon 2 was detected in 10 specimens. CONCLUSION： The finding suggested that there was little or no relationship between the homozygous deletion and point mutation of p16 gene and breast carcinogenesis.

关 键 词：乳腺癌 P16基因 纯合缺失 点突变 

分 类 号：R737.9[医药卫生—肿瘤] Q354[医药卫生—临床医学]