铅在不同基因型稻米中的富集  

Lead Enrichment in Different Genotypes of Rice Grains

作　　者：陈义芳 周卫东 刘爱平[2] 陈刚[2] 孙国荣[2] 

机构地区：[1]扬州大学测试中心,扬州225009 [2]扬州大学生物科学与技术学院,扬州225009

出　　处：《生物技术通报》2007年第1期128-132,共5页Biotechnology Bulletin

基　　金：国家自然科学基金(30270234)

摘　　要：通过环境扫描电镜结合X射线电子探针显微分析技术,对21种不同基因型水稻颖壳内、外表面以及糙米表面、糊粉层、近糊粉层和米中部Pb含量的测定结果表明,21种不同基因型稻米不同部位Pb含量的变化范围由大到小的顺序为:颖壳内表面>糊粉层>近糊粉层>糙米表面>颖壳外表面>米中部;不同基因型稻米之间对Pb的富集量存在遗传差异,这种差异体现在稻米的同一部位和不同部位之间Pb含量的差异上;不同基因型水稻米中部Pb含量与颖壳内表面、糊粉层、近糊粉层、糙米表面、颖壳外表面之间、水稻糊粉层Pb含量与颖壳内表面、近糊粉层、糙米表面、颖壳外表面、米中部之间以及颖壳内表面Pb含量与颖壳外表面、糊粉层、近糊粉层、糙米表面、米中部之间均存在极显著或显著的非线性关系。一方面反映出不同基因型稻米中部Pb的富集量均通过颖壳和糊粉层调控,即存在相同的调控机制;另一方面揭示了不同基因型稻米不同部位之间对Pb富集的相互调控能力存在差异,并且基因型间的这种差异存在非线性的变化规律。Using environmental scanning electron microscopy and X-ray electron probe microanalysis,the lead content was studied in inner and outer surface of rice glume,surface Of caryopsis,center of caryopsis,near aleuronic layer and aleuronic layer in 21 genotypes of rice grains. The results showed that the lead content in different part of 21 genotypes of rice grains changed as inner surface of rice glume〉aleuronic layer〉near aleuronic layer〉surface of caryopsis〉outer surface of rice glume〉center of caryopsis. There were genetic differences in lead enrichment in different genotypes of rice grains,which reflected as the differences of lead content in the same part and different part of rice grains. In different genotypes of rice grains,there were significant non-linear correlations between lead content in the center and that in inner surface of rice glume,aleuronic： layer,near aleuronic layer,surface of caryopsis and outer surface of rice glume,lead content in the aleuronic layer and that in inner surface of rice glume,near aleuronic layer,surface of caryopsis,outer surface of rice glume and center of caryopsis,lead content in the inner surface of rice glume and that aleuronic layer,near aleuronic layer,surface of caryopsis,outer surface of rice glume and center of caryopsis. The results also indicated that the lead enrichment in the center of caryopsis regulated by glume and aleuronic layer. In addition,in different genotypes of rice grains,there were differences in regulation of lead enrichment among different parts,which changed non-linearly.

关 键 词：稻米 铅 富集 X射线电子探针显微分析 遗传性 

分 类 号：X173[环境科学与工程—环境科学] S511[农业科学—作物学]