离子交换吸附L-色氨酸的研究  

Study on Adsorption of L-tryptophan by Ion Exchange

作　　者：刘芳[1] 谢丽敏[1] 沈忠耀[1] 

机构地区：[1]清华大学化工系,北京100084

出　　处：《生物工程学报》1996年第4期499-502,共4页Chinese Journal of Biotechnology

摘　　要：L-色氨酸是含吲哚环的芳香族氨基酸,也是人类必需的氨基酸之一,目前,工业上除部分采用化学合成法外,主要采用前体发酵法和酶促转化法生产。随着在医药、食品和饲料加工中应用的开拓,市场的需求量日益增加,色氨酸生产具有广阔的前途。Two cation exchangers were selected out for separating L-tryptophan from aqueous solution by comparing thrmodynamic and kinetic properties among fifteen ion-exchangers and adsorption resins. They had stronger adsorption capacity for L-tryptophan when pH was between 3 and 10. Dynamic test of cation exchanger showed that the saturattd adsorption capicity kept almost constants when the flow rate varied from 0. 04 to 0. 01 ml/ml - min. The adsorption efficiency achieved 99. 4% and the eluate was condensed 3. 68 times by using 1 mol/L ammonia solution at the flow rate from 0. 05 to ad-sorptin 0. 025 ml/ml - min. The total yield of L-tryptophan was 95% in the process.

关 键 词：L-氨基酸 发酵法 酶法 制备 离子交换吸附 

分 类 号：TQ922[轻工技术与工程—发酵工程] TQ93