超高压对枯草杆菌胞外蛋白酶的影响  

Effect of High Pressure on Exoproteinase of Bacillus subtilis

作　　者：谢慧明[1] 黄训端[1] 潘见[1] 曾庆梅[1] 王海翔[1] 蒋业雷[1] 

机构地区：[1]合肥工业大学农产品生物化工教育部工程研究中心,安徽合肥230009

出　　处：《高压物理学报》2007年第1期95-102,共8页Chinese Journal of High Pressure Physics

基　　金：农产品生物化工教育部重点实验室开放基金(NKF2004005);安徽省教育厅重点科研项目(2004kj004zd)

摘　　要：以枯草杆菌为研究对象,采用Box-Behnken响应曲面设计,研究了超高压处理对枯草杆菌胞外蛋白酶的影响以及菌体致死与胞外酶的关系。结果表明,超高压能显著抑制枯草杆菌胞外蛋白酶的活性,处理过程中压力和温度是酶失活的主要因素,并且两者之间存在交互作用,超高压处理时温度协同有效。利用Design Expert软件建立了胞外蛋白酶超高压钝化模型,决定系数R2=0.974 7,调整决定系数R2,adj=0.942 1。方差分析表明,模型显著,可应用于钝化效果分析与预测。实验结果还表明,超高压处理后枯草杆菌的致死率与胞外蛋白酶活的变化相关性达到极显著水平(R=0.933 0),胞外酶可作为超高压处理对微生物影响的一个评价指标。We studied the effect of high pressure process（HPP） on exoproteinase of Bacillus subtilis by using Box-Behnken response surface methodology. Experimental results showed that HPP could inhibit activity of exoproteinase significantly. In the course of treatment,pressure and temperature were key factors to passivate enzyme, and their interaction was notable. A model of exoproteinase passivation was established by design expert software,which determination coefficient was 0. 974 7 and adjusted determination coefficient was 0. 942 1. Analysis of variance implied that the model was significant. Therefore,the model could be used to analyze and predict. Additionally,the experiment indicated that exoproteinase activity correlated with lethal rate significantly（R=0. 933 0）. The exoproteinase could be regarded as an index to evaluate effect of HPP on microorganism.

关 键 词：超高压 枯草杆菌 胞外蛋白酶 响应曲面法 

分 类 号：O521.9[理学—高压高温物理] Q935[理学—物理]