辣椒素酯类物质的酶促合成  

Synthesis of capsinoids by lipase-catalysis

作　　者：李雨虹[1] 董新荣[2] 谢达平[1] 刘仲华[3] 

机构地区：[1]湖南农业大学生物科学与技术学院,湖南长沙410128 [2]湖南农业大学理学院,湖南长沙410128 [3]湖南农业大学天然产物研究中心,湖南长沙410128

出　　处：《化学研究与应用》2007年第4期427-429,共3页Chemical Research and Application

摘　　要：In this fig,natural capsinoids was synthesised from capsaicinoids by lipase-catalysis in acetone.The optimal reaction conditions as follows:50mmol vanillyl alcohol and 75mmol methyl nonanoate in 1 mL of acetone containing 0.1%～0.3% of water(V/V) in the presence of 20 mg lipase.Under the conditions,at 30℃,more than 60% of yield was achived after 9 h.The corresponding fatty acid methyl ester of capsaicinoids was obtained by menthanolysis of nature capsaicin in HCl methanol and then the capsinoids was gained by the lipase-catalyzed esterfication of vanillyl alcohol with corresponding fatty acid methyl ester in acetone.The products was purified by silica gel column chromatography and characterized including capsiate,dihydrocapsiate and nordihydrocapsiate in the compound by1HNMR and MS.In this fig, natural capsinoids was synthesised from capsaicinoids by lipase-catalysis in acetone. The optimal reaction conditions as follows ：50retool vanillyl alcohol and 75mmol methyl nonanoate in 1 mL of acetone containing 0. 1% - 0. 3% of water （V/V） in the presence of 20 mg lipase. Under the conditions, at 30℃ ,more than 60% of yield was achived after 9 h. The corresponding fatty acid methyl ester of capsaicinoids was obtained by menthanolysis of nature capsaicin in HC methanol and then the capsinoids was gained by the lipase-catalyzed esterfication of vanillyl alcohol with corresponding fatty acid methyl ester in acetone. The products was purified by silica gel column chromatography and characterized including capsiate, dihydrocapsiate and nordihydrocapsiate in the compound by^1 HNMR and MS.

关 键 词：辣椒素酯 脂肪酶 有机介质 酶催化 

分 类 号：O629.9[理学—有机化学]