纳豆菌原生质体制备与再生条件的研究  

Study on Conditions for Protoplast Production and Regeneration of Bacillus subtilis natto

作　　者：刘新梅[1] 高宇[2] 赵静[1] 李冰冰[1] 刘朔[1] 董明盛[1] 

机构地区：[1]南京农业大学食品科技学院 [2]南京理工大学科技处,江苏南京210094

出　　处：《食品科学》2007年第5期231-236,共6页Food Science

基　　金：国家"863"计划项目(2002AA248041);江苏高新技术研究项目(BG2002322)

摘　　要：研究了菌体培养时间、溶菌酶作用条件(酶作用温度、浓度和时间)对纳豆菌BNK菌株原生质体制备和再生的影响。在此基础之上,考察了不同再生培养基、渗透压稳定剂及原生质体保护剂对再生率的影响。确定了菌株BNK原生质体制备和再生最佳条件为:菌体培养9h收集,0．8mg/ml溶菌酶,30℃作用40min制备原生质体,将原生质体液涂布于以甘露醇为渗透压稳定剂、添加有淀粉的PYG培养基中再生,此时原生质体制备率达98．2%,再生率为28．4%。The effects of natto strain BNK cultivation time, operational times, temperature and concentration of lysozyme on protoplast production and regeneration were studied. In addition, different operational methods, regeneration media, osmotic stabilizers and protective additives were also investigated. The appropriate conditions of cultur are 9 h, BNK treated by 0.8 mg/ml lysozyme for 40 min at 30℃, then spreaded on PYG medium with mannitol as stabilizer and starch as a protector. Under these conditions the protoplast formation achieves 98.2% and regeneration frequency 28.4%.

关 键 词：纳豆菌 原生质体制备 再生 

分 类 号：Q939.97[生物学—微生物学]