牛乳中主要过敏原组分的分离纯化及鉴定  

Identification and Isolation of Major Allergens in Milk

作　　者：毛露甜[1] 向军俭[1] 张在军[1] 

机构地区：[1]暨南大学分子免疫学与抗体工程研究中心

出　　处：《食品科学》2007年第5期305-308,共4页Food Science

基　　金：广东省自然科学基金项目[2003(36708)];惠州学院校立项目(C_206.0209)

摘　　要：目的:分离纯化并鉴定牛乳中引起过敏反应的主要致敏组分。方法:采用聚丙烯酰胺凝胶电泳(SDS- PAGE)分析了脱脂乳和乳清的蛋白组分,用饱和硫酸铵分段盐析-DEAE离子交换柱层析纯化过敏原蛋白,脱脂乳和乳清蛋白皮下注射Balb/c小鼠获得高效价特异性IgE抗血清用于免疫印迹分析。结果:免疫印迹分析显示β-乳球蛋白(β-Lg)是引起牛乳过敏的主要过敏原,建立了牛乳过敏小鼠模型。结论:明确了牛乳过敏的主要过敏组分,对牛乳过敏症的诊断治疗以及无过敏原牛乳的制备提供了实验依据。Object： To isolate and identify the main allergens in the milk. Method： The protein components in skimmed milk and whey were analyzed by SDS-PAGE. The allergen proteins were purified by the combined of techniques saturated ammonium sulfate salirnetry and DEAE iron-exchanged chromatography. The Balb/c mice were immumed with the extracts of milk, then the high titrations of specific IgE anti-serum were isolated for immunoblotting analysis. Results： Immunoblotting analysis indicated that β-Lg is the major component leading to cow milk allergy. Allergic mice models were established successfully. Conclusion： The major allergic components in milk were identified in this study, paving an experiment basis for the diagnoses and therapy of milk allergey as well as the preparation of non-allergen milk.

关 键 词：牛乳 食物过敏原 免疫印迹 DEAE离子交换层析 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]