酶为介质有机溶剂提取万寿菊花中叶黄素的工艺研究  

Enzyme-mediated organic solvent extraction of lutein from marigold flower(Tagetes erecta)

作　　者：李大婧[1] 刘春泉[1] 方桂珍[2] 

机构地区：[1]江苏省农业科学院原子能农业利用研究所 [2]东北林业大学材料科学与工程学院,哈尔滨150040

出　　处：《农业工程学报》2007年第5期232-236,共5页Transactions of the Chinese Society of Agricultural Engineering

基　　金：江苏省科技攻关项目(BG2006319);江苏省农业科学院科研基金资助项目(6110537)

摘　　要：以万寿菊(Tagetes erecta)花粉末为原料,采用液态纤维素酶处理和有机溶剂萃取同时进行的方法提取叶黄素,研究并优化此过程的工艺条件。试验结果表明:酶为介质有机溶剂提取万寿菊花中叶黄素工艺条件受多种因素影响,其中提取时间、萃取前保温时间、酶浓度、搅拌速度对提取过程影响较大。确定酶法提取较适宜的工艺条件为:2.000 g粒度60目的万寿菊花粉末、液态纤维素酶浓度为7%(m/m)、液料比为20∶1、萃取前保温时间为1.5 h、搅拌速度为700 r/min、提取时间为3 h,对此工艺条件进行验证叶黄素提取率可达92.37%。而单纯的有机溶剂法叶黄素提取率仅为77.53%,此方法明显优于单纯的有机溶剂法。A technology for extracting lutein from marigold flower meal was studied by using simultaneous cellulolytic enzyme treatment and organic solvent extraction methods. The optimal conditions in the enzymemediated organic solvent extraction process were investigated. The results show that the primary factors which affected the enzyme-mediated organic solvent extraction are extraction time, incubation time previous to extraction, enzyme concentration and agitation rate in the extraction process in comparison with the others. The optimal conditions are as follows： 2. 000 g 60 mesh marigold flower meal as material, 7%（m/m, flower meal） liquid cellulase concentration for 1.5 h incubation and 3 h extraction duration with 700 r/min agitation rate in combination with 20 ： 1 （volume/weight） solvent to solid weight ratio, under which high extraction yield（92. 370%） of lutein can be achieved by means of verification test. In addition, the extraction yield of lutein extracted by using single organic solvent is just 77.53% and the results suggest that the enzyme-mediated organic solvent extraction process is superior to single organic solvent extration method.

关 键 词：万寿菊花 叶黄素 纤维素酶 提取工艺 

分 类 号：TS201.1[轻工技术与工程—食品科学] Q562[轻工技术与工程—食品科学与工程]