米根霉丙烯醇抗性突变株的筛选及其发酵特性  

SCREENING AND FERMENTATION CHARACTERIZATION OF RHIZOPUS ORYZAE ALLYL ALCOHOL RESISTANT MUTANT

作　　者：郑志[1] 姜绍通[1] 罗水忠[1] 李兴江[1] 潘丽军[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《核农学报》2007年第3期242-245,255,共5页Journal of Nuclear Agricultural Sciences

基　　金：教育部博士点基金项目资助(20040359008);合肥工业大学科学研究发展基金资助项目(063001F)

摘　　要：本研究利用亚硝基胍(NTG)对米根霉As3.3462进行诱变,在含丙烯醇的YPD选择培养基上筛选获得11株ADH活力降低的突变株,其中mut-2突变株发酵液中乙醇含量比原始菌株降低35.2%,而乳酸含量提高了79.6%。对原始菌株和mut-2突变菌株在发酵过程中ADH与LDH活力变化分析表明,突变菌株的最大ADH活力比原始菌株降低了60.6%,而LDH活力与原始菌株相比略有提高。研究结果还表明,mut-2突变菌株与出发菌株相比,其生物量和对还原糖的利用速率均比原始菌株提高。Rhizopus oryzae As3.3462 was mutated with N-methyl-N＇-nitro-N-nitrosoguanidine （NTG） and 11 allyl alcohol resistant mutants were obtained with selective medium of yeast-peptone-dextrose （YPD） containing 0.6 % allyl alcohol （V/V）. Among these mutants, mut-2 had the highest conversion rate of 75.2g/L to L-lactic acid, increased 79.6% compared with wild type strain, and conversion rate to ethanol decreased 35.2%. The comparisons of ADH specific activity and lactate dehydrogenase （LDH） specific activity between the mutant and wild type strain during the fermentation showed that rout-2 had a markedly lower ADH specific activity and an appreciably higher LDH specific activity than wild type strain. The mut-2 also had higher consumption rate of glucose and biomass accumulation than wild type.

关 键 词：米根霉 L-乳酸 乙醇 ADH 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]