酪蛋白非磷肽分子组成及其抑制血管紧张素转换酶活性的研究  

STUDY ON THE MOLECULAR COMPOSITION AND ANGIOTENSIN-CONVERTING ENZYME INHIBITION ACTIVITY OF CASEIN NON-PHOSPHOPEPTIDE

作　　者：赵利[1] 王璋[2] 许时婴[2] 杨玉玲[3] 王素雅[3] 

机构地区：[1]江西科技师范学院生命科学学院,江西南昌330013 [2]江南大学食品学院,江苏无锡214036 [3]南京财经大学食品科学与工程学院,江苏南京210003

出　　处：《河南工业大学学报（自然科学版）》2007年第3期76-80,共5页Journal of Henan University of Technology：Natural Science Edition

摘　　要：测定了酪蛋白非磷肽（CNPP）的分子组成，并对酪蛋白非磷肽抑制血管紧张素转化酶（ACE）的活性进行了研究，研究结果表明：酪蛋白非磷肽的相对分子质量分布在226～8314之间，其中数均相对分子质量253的肽含量最多，约占26.54％，其次是数均相对分子质量603的肽，含量约占25.69％；CNPP具有较强的疏水性，其中碱性氨基酸Lys、His和Arg的含量为11.29％，芳香族氨基酸Tyr和Phe的含量为13.03％；CNPP对ACE活性具有抑制作用，且具有一定的量效关系，IC50是0.63μmol／L。The molecular composition and Aiagiotensin-converting enzyme （ACE） inhibition acticity of Casein non-phosphopeptides （CNPP） were determined using HPLC. The result showed that the relative molecular weight of CNPP was in the range of 226～8314,the peptides with number-average molecular weight of 253 re-presented 26.54%, and the peptides with number-average molecular weight of 603 represented 25.69%;CNPP possess the property of high hydrophobic, there were 11.29% alkaline amino acid Lys, His, Arg and 13.03% aromatic amino acid Tyr, Phe in them; CNPP had the function of ACE inhibition, the inhibition was increased with increasing of the concentration of CNPP and IC50 was 0.63 μmol/L.

关 键 词：酪蛋白非磷肽 血管紧张素转化酶 抑制 

分 类 号：TQ93[化学工程]