壳寡糖的酶法制备  

Preparation of Chitooligosaccharides by Enzyme Hydrolysis

作　　者：陈小娥[1] 方旭波[1] 夏文水[2] 

机构地区：[1]浙江海洋学院食品与药学学院,浙江舟山316000 [2]江南大学食品学院,江苏无锡214036

出　　处：《功能高分子学报》2007年第2期204-208,共5页Journal of Functional Polymers

基　　金：浙江省自然科学基金资助项目(Y305119)

摘　　要：通过还原端基、酶解产物聚合度、水解液调碱性后透光率的测定以及酶解产物的TLC、HPLC分析，研究了壳聚糖酶降解壳聚糖过程中壳聚糖浓度、酶用量、反应时间和pH对酶促反应速率和产物聚合度的影响。结果表明：壳聚糖酶在底物浓度0．03g／mL、壳聚糖酶用量4～6u／g、pH=5．8和水解200rain时，可得到以聚合度3～7为主的壳寡糖。Through determination the reducing sugar , average polymerization degree , transmittancy of chitosan hydrolysis products, the effect of chitosan concentration, temperature, pH, and quantity of enzyme to the enzyme hydrolysis were investigated. The results show that the optimum enzyme hydrolysis condition is pH= 5.8, 3G of substrate concentration, and enzyme content of 4 to 6 u/g chitosan, respectively. Polymerization degree of 3～7 are obtained after chitosan is degradated by the chitoanase for 200 min.

关 键 词：壳聚糖 壳聚糖酶 水解 壳寡糖 

分 类 号：O631[理学—高分子化学]