菌株N235产海藻糖合酶工艺条件的优化  

Optimization of conditions on liquid fermentation of trehalose synthase enzyme

作　　者：宁维颖[1] 鲍晓明[2] 王瑞明[1] 郭利美[1] 

机构地区：[1]山东轻工业学院食品与生物工程学院,山东济南250353 [2]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《山东轻工业学院学报（自然科学版）》2007年第2期74-76,80,共4页Journal of Shandong Polytechnic University

基　　金：山东省优秀中青年科学家基金资助项目(BS0384)

摘　　要：通过对筛选得到的海藻糖产生菌N235（Pseudomonas sp）的培养温度、初始pH、溶氧量、接种量、渗透压、培养时间等参数对菌体生物量和单位菌体海藻糖合酶酶活力的影响的研究，得到了最佳酶活培养条件为：培养温度35℃、培养基初始pH5-6、装液量100mL／500mL三角瓶、渗透压浓度为5％NaCl、培养时间为64h，在此条件下，酶活达到252U／g干菌体。The fermentation conditions of N235（ Pseudomonas sp）were studied. The conditions include medium volume, initial pH, temperature and time affected on biomass and trehalose synthase enzymes activities. The result showed that the optimal culture condition was 10% spores, 100 mL medium volumn were inculated in 500 mL Erlenmeyer-flask, initial pH 5 - 6, temperature 36 ℃ for 64 h. Under the optimized conditions, the biomass was 18.50 g,/L and the enzyme activity is 252.0 U/g dry thalli.

关 键 词：海藻糖合酶 32艺条件 优化 假单胞菌 

分 类 号：Q559[生物学—生物化学]