HPLC测定鸡腿蘑发酵液中4,5-二羟基-2-甲氧基苯甲醛  

Determination of 4,5-Dihydroxy-2-Methoxy-Benzaldehyde in Coprinus comatus fermentation broth by high performance liquid chromatography

作　　者：丁重阳[1] 陆兆新[1] 陶冠军 王玉红[3] 章克昌[3] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]江南大学分析测试中心,江苏无锡214122 [3]江南大学教育部工业生物技术重点实验室,江苏无锡214122

出　　处：《江苏大学学报（自然科学版）》2007年第4期277-279,共3页Journal of Jiangsu University：Natural Science Edition

基　　金：教育部科技基础条件平台建设计划资助项目(505006)

摘　　要：以鸡腿蘑发酵液中分离获得的具有抑制蛋白质非酶糖基化活性的新物质4,5-二羟基-2-甲氧基苯甲醛为对象,建立检测鸡腿蘑发酵液中该物质的高效液相色谱方法.其回归方程为Y=675.42X-943.39(r2=0.999 0),线性范围为1.23～123 mg/L.该方法的日内和日间精度分别为1.5%～5.6%,2.5%～4.6%,回收率为93.8%～96.6%.该方法能够在20 m in内完成一次分析,具有快速、准确、可靠的特点.4,5-Dihydroxy-2-Methoxy-Benzaldehyde（4,5-DHMB）, a novel natural compound with inhibition of non-enzymatic glycosylation, was obtained from Coprinus comatus fermentation broth. A high per- formance liquid chromatographic procedure was developed and validated for the determination of 4,5-DHMB in Coprinus comatus fermentation broth. Chromatographic separation is performed on a C18 column （150 mm ×2.1 mm i. d. , 5 μm particle）. The mobile phase is composed of methanol-water （30： 70） to 100% methanol in 20 min for gradient elution at a flow rate of 0.3 mL/min. The method has good linear range of 1.23 ～ 123 mg/L with r2 =0. 999 0. Intra- and inter-day precision ranged from 1.5% to 5.6% and 2.5% to 4.6%, respectively. The recovery was within 93.8% ～ 96.6%. The assay is simple, quick and precise, which assures the continuous research for Coprinus comatus liquid fermentation and 4, 5-DHMB.

关 键 词：4  5-二羟基-2-甲氧基苯甲醛 高效液相色谱 鸡腿蘑 液体发酵 非酶糖基化反应 

分 类 号：TS201.1[轻工技术与工程—食品科学] O657.7[轻工技术与工程—食品科学与工程]