大豆胚轴中DDMP皂甙βg的纯化及其清除DPPH自由基能力  

Purification of DDMP saponin βg from soybean hypocotyl and its scavenging capacity against DPPH radical

作　　者：全吉淑[1] 沈明花[1] 陈丽艳[1] 尹学哲[1] 

机构地区：[1]延边大学医学院,吉林延吉133000

出　　处：《食品工业科技》2007年第6期59-61,共3页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(30360113)。

摘　　要：从大豆胚轴提取总糖甙,以C18柱层析法和高效液相色谱法(HPLC)分离纯化DDMP皂甙βg,并以比色法检测对1,1-二苯基-2-苦味酰(DPPH)自由基的清除能力。结果表明,DDMP皂甙βg具有很强的清除DPPH自由基能力,其半抑制浓度(IC50)值为51μmol/L。Total glycosides were extracted from soybean hypocotyl, and DDMP saponin βg were isolated with C18 column chromatography and high-performance liquid chromatography （HPLC）, and scavenging activity against 1,1 -diphenyl -2 -picrlhydrazyl （DPPH） radical was then tested by colorimetric method. DDMP saponin βg showed potent scavenging activity against DPPH radical with IC50 value of 51μmol/L.

关 键 词：大豆胚轴 大豆皂甙 DPPH自由基 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]