高效液相色谱法测定高含水量辣椒制品中的辣椒素类物质  

Determination of Capsaiciniods in High Water-bearing Pepper Products by High Performance Liquid Chromatography

作　　者：王穗萍[1] 夏延斌[1] 彭进[2] 熊科[1] 郑瑶瑶[1] 杨峰[1] 

机构地区：[1]湖南农业大学食品科技学院,湖南长沙410000 [2]常德市疾病预防控制中心,湖南常德415000

出　　处：《分析测试学报》2007年第4期570-573,576,共5页Journal of Instrumental Analysis

基　　金：农业部948专项经费资助项目(2003T18)

摘　　要：样品以70％乙醇提取，采用反相高效液相色谱分离，色谱条件：色谱柱ZORBAX SB C184．6mm×250mm，5μm；流动相为甲醇-水（体积比70：30）；流速0．8mL／min；UV230nm检测；进样量20μL。以外标峰面积法定量辣椒素（C）、二氢辣椒素（DC）、去甲二氢辣椒素（NDC），结果以斯氏指数（SHU）计：NDC的色谱行为与C极其类似，NDC采用C的标准曲线定量。C和DC的RSD分别为1．0％、1．5％（n=5）；平均回收率分别为97％、96％：C和DC在0．001～0．1g／L范围内线性良好；检出限（LOD）为0．1mg／L，定量下限（LOQ）为0．5mg／L。方法满足辣味水平评价要求。A method was developed for the determination of eapsaieinoids in highly water-bearing pepper products by high performance liquid chromatography. The sample was extracted with 70% ethanol. The extract was separated on a ZORBAX SB C18 column（250 ×4. 6 mm, i. d. 5 μm） at 30℃ , which was then eluted with 70% MeOH aqueous solution at a flow rate of O, 8 mL,/min, The UV detection was at λ230 nm With the external standard method, the linear range of the plots of the peak area versus the concentration for eapsaiein （ C ） , dihydroeapsaiein （ DC ） and nordihydroeapsaiein （ NDC ） , respectively were in the range of O. 001 -0. 1 g/L with a correlation coefficient of 0, 999 8, The determination results were presented in Seoville heat unit. Since the chromatographic behavior of NDC and C were similar, NDC could be determined using the plot of C, The limits of detection and limits of quantification for C and DC were 0, 1 mg/L and 0. 5 mg/L, respectively. The relative standard deviations for C and DC were 1.0% and 1.5% respectively（n =5）and the average recoveries were 97% and 96% respectively, Based on the developed method, the pungency level for the pepper products could be measured accurately,

关 键 词：高效液相色谱 高含水量 辣椒制品 辣椒素同系物 辣味水平 

分 类 号：O657.72[理学—分析化学] S641.3[理学—化学]