基因工程菊粉酶对高浓度蔗糖水解的研究  

Study on gene engineering inulinase hydrolyzed hypso-concentration sucrose

作　　者：常晓川[1] 郑昕[2] 王运吉[1] 张苓花[2] 

机构地区：[1]大连轻工业学院生物与食品工程学院,大连116034 [2]大连海事大学,大连116026

出　　处：《食品科技》2007年第7期76-79,共4页Food Science and Technology

基　　金：辽宁省教育厅资助项目(202073415)。

摘　　要：对菊粉酶基因工程菌株GS115/INU1进行诱导表达,通过发酵液的分离纯化后,得到在SDS电泳显示单一条带的GS115/INU1酶液。该酶的蔗糖水解酶比活性为1.85U/μg,水解蔗糖最适pH值为4.0,最适温度50℃。加酶量为48U/g底物,对50%蔗糖溶液进行水解,水解时间6h,水解率达到99.7%。对80%蔗糖溶液进行水解,水解时间14h,水解率达到97.8%。The recombinant inulinase strain GS115/INU1 was induced and expressed. After broth separation and purification, enzyme liquid of GS115/INU1 was obtained which presented single zone in SDS electrophoresis. The optimum temperature and pH of the sucrose hydrolysis were at 50℃ and pH4.0, respectively.Under 48U enzyme per gram sucrose condition, the enzyme hydrolyzed sucrose solution at concentration 50% for 6 hours, and the hydrolysis rate approached 99.7%. The enzyme hydrolyzed sucrose solution at concentration 80% for 14 hours, the hydrolysis rate approached 97.8%.

关 键 词：果葡糖浆 基因工程菊粉酶 水解率 高浓度 

分 类 号：TS201[轻工技术与工程—食品科学]