甘蔗糖厂混合汁浮渣总黄酮提取工艺的研究  

Study on extraction conditions of total flavonoids from the mixed juice scum of sugarcane mill

作　　者：韩忠[1] 陈山[1] 袁竹连[1] 莫海涛[1] 刘丽娅[1] 王军[1] 

机构地区：[1]广西大学糖业工程技术研究中心,南宁530004

出　　处：《食品科技》2007年第7期138-141,共4页Food Science and Technology

基　　金：国家"科技型中小企业技术创新基金"项目(06C26224501690);广西教育厅项目(200420)

摘　　要：以不同浓度的乙醇、料液比、超声波提取温度及提取时间等条件对甘蔗糖厂混合汁浮渣总黄酮的提取进行正交设计试验,并以芦丁为标准品,用分光光度法在510nm处测定各提取液的总黄酮含量。结果显示:超声波乙醇提取法最佳工艺组合为A2B2C3D2,即乙醇浓度60%,料液比1∶50,超声波提取温度80℃,超声波提取时间60min。The flavonids in the mixed juice scum of sugarcane mill, were extracted by different extraction conditions based on the orthogonal experiments design. Rutin was used as a standard compound and flavonoids content of the mixed juice scum was quantified by measuring the absorbance at 510nm by spectrometric method. The results indicated the optimum extracting condition of ultrasonic intensified ethanol extraction method being A2B2C3D2, i.e., 60% ethanol as solvent, ratio of liquid/solid as 1：50, temperature of extraction at 80℃ and extracting time of 60min.

关 键 词：混合汁浮渣 分光光度法 总黄酮 提取工艺 

分 类 号：TS201.1[轻工技术与工程—食品科学]