鸡蛋黄中磷脂的提取及分析  

Extraction and Analysis of Phospholipids in Egg Yolk

作　　者：张佑红[1] 陈燕[1] 朱雄伟[1] 秦琴[1] 吴元欣[1] 杜进[2] 谈雅静[3] 候畅[3] 赵亮[1] 吕中[1] 

机构地区：[1]武汉工程大学湖北省新型反应器与绿色化学工艺重点实验室,湖北武汉430073 [2]北京大学医学部,北京100083 [3]武汉化工学院,湖北武汉430023

出　　处：《食品科学》2007年第7期373-376,共4页Food Science

基　　金：湖北省重点实验室开放基金项目(RCT2004004);武汉市科技攻关项目(20052002044)

摘　　要：本研究利用磷脂在丙酮中的相对不溶性,将磷脂从蛋黄中提取出来,用分光光法在630nm下测量蛋黄中磷脂的含量。然后以纸层析为主要的定性检测手段来检测磷脂X基团,用薄层色谱法对磷脂混合物的各个成分进行定性分析。Based on relative indissolubility of phospholipid in acetone,phospholipids in egg yolk were extracted in this research. The content of phosphorus was determined with spectrophometry at 630rim. Group X in phospholipid was detected by paper chromatography. At least four fractions of the phospholipids were separated by thin layer chromagraphy and the phenomena was explained.

关 键 词：蛋黄磷脂 提取 纸层析 薄层层析 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程]