复合酶解乳清蛋白制备降血压肽的研究  

Preparation of angiotensin I converting enzyme inhibition from whey protein by double enzymes

作　　者：包怡红[1] 徐思源[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《中国乳品工业》2007年第7期25-28,共4页China Dairy Industry

基　　金：哈尔滨市科委青年基金项目。

摘　　要：采用碱性蛋白酶、胰蛋白酶水解乳清蛋白制备ACE抑制肽,通过体外检测法测定其ACE抑制率。通过正交试验,得出最优水解条件,即碱性蛋白酶和胰蛋白酶[E1/E2]之比为5:4,温度为45℃,pH值为8.0,时间为150min,水解度11.92%的条件下,乳清蛋白肽对ACE的抑制能力最强,达到60.19%。ACE inhibitory peptides were prepared from whey protein by composite enzyme （alcalase, trypsin） and the ACE inhibitory activity were determined in vitro by spectrophotometry assay. The optimum conditions of enzymic hydrolysis got by orthogonal experiment were [E1/ E2]（ （alcalase：trypsin）=5 ： 4, temperature=45℃, pH=8.0 for 150 min, and DH=11.92%, the most ACE inhibitory activity of hydroxyl radical can be approached to 60.19%.

关 键 词：乳清蛋白 ACE抑制肽 ACE抑制活性 双酶 

分 类 号：Q516[生物学—生物化学]