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机构地区:[1]邵阳学院生物与化学工程系,湖南邵阳422000
出 处:《酿酒科技》2007年第8期144-145,149,共3页Liquor-Making Science & Technology
基 金:湖南省教育厅资助科研项目(编号04C606)。
摘 要:将白地霉接种在添加亚硒酸钠的培养液中进行驯化,以大曲丢糟为主要原料,以富硒白地霉为菌种,适当添加纤维素酶进行富硒饲料蛋白的发酵生产,通过正交试验得到最佳的饲料生产工艺条件为:Na2SeO3添加量为0.50mg/kg,富硒白地霉接种量为15%,纤维素酶添加量为0.03%,培养时间4d。所生产得的富硒饲料粗蛋白和有机硒含量分别达到了18.9%、0.18mg/kg。Geotrichum candidium link was inoculated in the culture solution (Na2SeO3 was added) for domestication, then Daqu distiller's grain was used as the main raw materials and Se-enriched geotrichum candidiurn link was used as microbial species to produce Se-enriched feed protein by the addition of cellulase. The best technical conditions were summed up through orthogonal test as follows: the addition level of Na2SeO3 was 0.50 mg/kg,the inoculating quantity of Se-riched Geotrichum candidium link was 15 %, the addition level of cellulase was 0.03 % and the culture time was 4 d. The content of rough protein and organic-Se in the produced feed protein increased up to 18.9 % and 0.18 mg/kg respectively.
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