太空搭载对短双歧杆菌A04体外抗氧化能力的影响  

Effects of Spaceship-carried Induction on Antioxidant Activity of Bifidobacterium breve A04 in vitro

作　　者：张博文[1] 张肃[1] 雷浪伟[2] 王延祥[1] 陈斌[2] 李平兰[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国航天员科研训练中心,北京100094

出　　处：《食品科学》2007年第8期261-266,共6页Food Science

基　　金：中国航空航天医学研究所基金项目(SJ200607);国家"十一五"科技支撑项目(2006BAD04A06);国家"863"目标导向性项目(2006AA10Z343)

摘　　要：为了研究太空搭载对长寿老人源短双歧杆菌A04(B.breveA04)体外抗氧化能力的影响,本实验以长寿老人源短双歧杆菌A04以及神州6号飞船搭载后的短双歧杆菌A04菌株为研究对象,进行了抗脂质过氧化试验、还原力测定试验、铁离子螯合试验、清除DPPH自由基试验、电子共振自旋捕集(ESR)法检测清除羟自由基和超氧阴离子的能力试验,并对其体外抗氧化能力进行了比较研究。实验结果表明,长寿老人源短双歧杆菌A04在太空搭载前后均具有较好的抗氧化活性,并且经太空搭载后,无论是活菌体还是菌体无细胞提取物,搭载后的短双歧杆菌A04菌株的抗氧化活性都得到了一定程度的提高。In order to study the effects of spaceship-carried induction on the antioxidant activity of B.breve A04 in vitro, inhibition of linoleic acid peroxidation, reducing power, chelating ability, l, 1-diphenyl-2-picryhydrazyl （DPPH） free radical scavenging ability, hydroxyl radical and reactive oxygen species scavenging activity of B.breve A04 and B.breve HA04 were experimentized. The results showed that both the B.breve A04 and the B.breve HA04 have strong activity of antioxidation. And after compared with the antioxidant activity ofB.breve A04 and B.breve HA04, we found that the antioxidant activity of B.breve HA04 was stronger than that ofB.breve A04. It was indicated the antioxidant activity ofB.breve A04 was enhanced after spaceship- carded.

关 键 词：双歧杆菌 太空搭载:体外抗氧化:清除自由基能力 

分 类 号：R378.2[医药卫生—病原生物学]