龙须菜藻红蛋白抗肿瘤活性的研究  

Studies on antitumor activities of phycoerythrin from Gracilaria lemaneiftheormis

作　　者：陈美珍[1] 葛安山[1] 余杰[1] 杨文杰[1] 

机构地区：[1]汕头大学理学院生物系,广东汕头515063

出　　处：《中国海洋药物》2007年第4期27-31,共5页Chinese Journal of Marine Drugs

基　　金：汕头市科技计划资助项目(2006-149)

摘　　要：目的探讨龙须菜藻红蛋白抗肿瘤活性。方法体内实验以S180荷瘤小鼠为肿瘤模型，检测藻红蛋白粗提物（CPE）对肿瘤生长的抑制作用以及对免疫器官的影响；体外实验采用MTT法检测不同浓度的藻红蛋白（PE）对人宫颈癌HeLa、人食道癌EC109和人血管内皮细胞等细胞的作用，流式细胞仪、Annexin V-FITC／PI双荧光染色法观察藻红蛋白对HeLa细胞周期及细胞凋亡的影响。结果CPE能显著的抑制小鼠S180肉瘤生长，当灌胃剂量在100～300mg·kg^-1范围内时，呈量效关系，其中剂量为300mg·kg^-1时，其抑瘤率达49％，且能极显著地提高荷瘤小鼠的胸腺指数；体外实验表明PE对人宫颈癌细胞HeLa有明显的抑制作用，其抑制率达70％以上，并存在剂量依赖性。PE可阻滞HeLa细胞从G2／M期进入S期，促使细胞凋亡。但PE对人食道癌EC109细胞无作用，对正常的人血管内皮细胞有一定的促生长作用。结论龙须菜藻红蛋白具有体内抑制S180肉瘤生长的活性，体外对培养的不同细胞作用不同，对人宫颈癌HeLa细胞有较强的抑制作用。Objective To investigate the antitumor effects of phycoerythrin from Gracilaria lemaneiftheormis. Methods The coarse phycoerythrin （CPE） was ig administered to sarcorea180 mice, then the antitumor effect of CPE in vivo was evaluated by the inhibition rate, thymus and spleen indexes. The growth inhibitory effect of phycoerythrin （PE） on cell lines HeLa, EC109 , and vascular endothelial in vitro was examined with MTT assay. Influences on HeLa cell cycle and apoptosis of PE were observed by flow cytometry and annexin v-fitc/ PI fluorescence staining. Results CPE exhibited inhibitory effect against sarcoma180 growth in mice at the dosage of 100~300 mg·kg^-1with a fair dose-effect relationship, and the inhibitory rate was 49.23% at the dosage of 300 mg · kg^-1. CPE could also obviously increase the weight of thymus; PE could obviously inhibit the growth of HeLa cell in a dose-effect relationship, the inhibitory rate could reach more than 70%. It could prevent the transforming of HeLa cell cycle from G2/M to S phase to cause apoptosis. But PE couldn＇t inhibit the growth of EC109 cell, while some growth stimulating effects of PE on vascular endothelial cell were observed. Conclusion The PE from Gracilaria lemaneiftheormis could observably inhibit sarcomas80 growth in vivo. Effects of PE on different cells in vitro were differ, but showed potent inhibitory effect to HeLa cell.

关 键 词：龙须菜 藻红蛋白 S180肉瘤 HELA细胞 

分 类 号：R931.711[医药卫生—生药学] R965[医药卫生—药学]