黄粉虫中提取纯化超氧化歧化酶的工艺研究  

Study on Technology of Extraction and Purification Superoxide Dismutase from Yellow Mealworm

作　　者：吴蕾[1] 陈庆森[1] 刘晋生[2] 

机构地区：[1]天津市食品生物技术重点实验室,天津商业大学生物工程系,天津300134 [2]天津大学化工学院生物工程系,天津300072

出　　处：《食品科学》2007年第9期281-283,共3页Food Science

基　　金：天津市科技攻关计划重点专项项目(06YFGZNC04200)

摘　　要：本研究以黄粉虫粉为原料,经盐析沉淀,DEAE-Sepharose FF阴离子交换柱层析和Sephadex G200凝胶过滤层析等纯化步骤,获得了其中的一种超氧化物歧化酶(SOD)。酶的纯化倍数为78.3倍。SDS-PAGE电泳显示为均一的蛋白谱带。经鉴定该酶为Cu.Zn-SOD。本研究为从黄粉虫中提取SOD提供了一种较好的工艺。In this paper, a kind of superoxide dismutase （SOD） was obtained from yellow worm powder. Two-step（NH4）2SO4 precipitation were used as the main extraction method and DEAE-Sepharose FF ion-exchange and Sephadex G200 volume exclude chromatography were used as purification methods, The purification factor was 78.3.The SDS-PAGE showed that the SOD was a homogenous protein. This enzyme was proved as Cu.Zn-SOD. This study provides a suitable technology for obtaining SOD from the yellow mealworm.

关 键 词：黄粉虫 超氧化歧化酶 提取 纯化 柱层析 

分 类 号：Q814.4[生物学—生物工程]