龙须菜藻红蛋白对Hela细胞增殖抑制及其机制的研究  

Study on Inhibition Effects of Phycoerythrin from Gracilaria lemaneiformis on Hela Cells and Its Mechanism

作　　者：陈美珍[1] 葛安山[1] 崔鹏举[1] 廖灶辉[1] 

机构地区：[1]汕头大学理学院生物系,广东汕头515063

出　　处：《食品科学》2007年第9期549-552,共4页Food Science

基　　金：汕头市科技计划资助项目(2006-149)

摘　　要：目的:探讨龙须菜藻红蛋白(PE)对人宫颈癌细胞Hela体外的抑制作用及其作用机制。方法:采用MTT法检测不同浓度的藻红蛋白对Hela细胞增殖抑制效果。流式细胞仪和Annexin V-FITC/PI双荧光染色法观察藻红蛋白对Hela细胞周期及细胞凋亡的影响。结果:PE可显著抑制Hela细胞的生长,并呈剂量-效应关系(p<0.01,r=0.84),剂量为20μg/ml,作用48h,其抑制率达70.88%,其48h的IC50值为4.12μg/ml。PE可阻滞Hela细胞从G2/M期进入S期,诱导细胞凋亡。结论:PE对Hela细胞有较强的抑制作用,其作用机制部分与诱导Hela细胞调亡有关。Objective： To investigate the inhibition effects of phycoerythrin （PE） from Gracilaria lemaneiftheormis on growth of Hela cells and its mechanism. Method： The proliferation of cell lines of Hela in vitro was assayed by MTT method. Effects of PE on Hela cells cycle and apoptosis were observed by flow cytometry and Annexin V-FITC/PI fluorescence staining. Result： PE could obviously inhibit the proliferation of Hela cells with a fair dose-effect relationship （p 〈 0.01, r =0.84）. The inhibitory rate at 48 h could reach more than 70% and its half inhibitory concentration （IC50） was 4.12 μg/ml. PE could prevent the transformation of Hela cell cycle from G2/M to S phase to cause apoptosis. Conclusion： PE shows potent inhibitory effects on growth of Hela cells, and the mechanism is related to inducing the apoptosis of Hela cells.

关 键 词：龙须菜藻红蛋白 HELA细胞 细胞调亡 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]