取材时期和BA浓度对磨盘柿初代组培建立的影响  

The effect of sampling time and concentration of BA on culture establishment of Mopan persimmon

作　　者：宋春丽[1] 马俊莲[2] 刘月英[3] 周平平[1] 徐立强[2] 

机构地区：[1]河北农业大学中兽医学院,河北定州073000 [2]河北农业大学食品科技学院,河北保定071001 [3]河北经贸大学生物科学与工程学院,河北石家庄050061

出　　处：《河北林果研究》2007年第3期309-313,共5页Hebei Journal of Forestry and Orchard Research

基　　金：河北省科技厅项目(303196)

摘　　要：以磨盘柿休眠芽、萌动芽或梢尖为外植体,研究了取材时期和BA浓度对磨盘柿初代组培建立的影响。结果表明,1-3月上旬、9-12月休眠芽外植体萌芽率达80%以上,且增殖能力强,为取材最佳时期,4、5月份梢尖褐变死亡率达80%以上,不宜建立初代培养;BA5.0 mg/L适于磨盘柿初代组培建立;培养基中附加500mg/L的聚乙烯吡咯烷酮（PVP-40）可有效防止休眠芽外植体褐变。The effect of culture establishment with buds or young shoots taken at different time was compared. Of test tube. plantlets the effect of different concentration of BA were discussed. The main results were as follows： 1. Necrosis of actively growing shoots tips was over 80%, which limited the culture establishment. Compared with shooting tips, buds taken in January to March or September to December were the much better explants, of which more than 80% survived and started growing in the first generation. 2. The culture of Mopan persimmon could be established in medium with BAS. 0- 7.5 mg/L. 3. PVP - 40 500 mg/L could prevent dormant bud from browning in the process of tissue culture.

关 键 词：柿 离体快繁 取材时期 褐变 

分 类 号：S665.2[农业科学—果树学]