细菌产β-葡萄糖苷酶发酵优化  

Optimization of Fermentation Conditions for Producing β-Glucosidase with Bacterium

作　　者：方尚玲[1] 刘源才 张庆华[1] 林英[1] 陈道玉 李世杰[1] 

机构地区：[1]湖北工业大学工业微生物湖北省重点实验室,湖北武汉430068 [2]湖北劲牌集团公司,湖北大冶435100

出　　处：《化学与生物工程》2007年第9期54-58,共5页Chemistry & Bioengineering

摘　　要：从栀子果中分离到一株在较高温度下产β-葡萄糖苷酶的地衣芽孢杆菌,对其产酶条件进行了优化。通过单因素实验和正交实验确定该菌株产酶的最佳培养条件为:碳源(甘蔗渣粉∶麸皮=3∶1)5%,牛肉膏0.5%,栀子甙0.2%,KH2PO40.4%,MnSO40.04%,pH值9.0,装液量20 mL/250 mL,种子液接种量10%,45℃发酵24 h。优化条件下发酵液中的酶活可达到93.48 U.mL-1。A strain of Bacillus licheniformis isolated from gardenia fruits was used to produce the β-glucosidase at high temperature. The optimum fermentation conditions obtained by individual factor tests and orthogonal tests were as follows.carbon source（sugarcane powder ： wheat bran=3 ： 1）5% ,beef extract 0. 5% ,geniposide 0. 2%,KH2PO4 0. 4%,MnSO4 0. 04%,pH value 9. 0,liquid volume in the flask 20 mL · （250 mL）^-1,inoculating amount 10% ,cultivation temperature 45℃, cultivation time 24 h. Under optimum conditions, the activity of β-glucosidase was 93.48 U · mL^-1.

关 键 词：Β-葡萄糖苷酶 地衣芽孢杆菌 发酵 优化 

分 类 号：TQ925[轻工技术与工程—发酵工程] Q93-335[生物学—微生物学]