50L自控式发酵罐中ε-聚赖氨酸发酵条件的优化  

Optimization of the ε-Poly-L-lysine fermentation conditionsin 50L autocontrol fermentor

作　　者：黄国昌[1] 印培民[2] 黄筱萍[2] 

机构地区：[1]南昌大学生命科学院,江西南昌330047 [2]江西省科学院,江西南昌330029

出　　处：《中国食品添加剂》2007年第5期99-103,98,共6页China Food Additives

摘　　要：在50L自控式发酵罐上进行ε-聚赖氨酸发酵条件优化研究,主要考察了分批发酵和补料分批发酵过程中pH、搅拌转速对ε-PL发酵和菌体生长的影响。结果表明,当控制pH在5.0以上时有利于菌体的生长,但ε-聚赖氨酸基本不合成,甚至出现降解现象;当pH维持在4.0左右时,能促进ε-聚赖氨酸的合成。搅拌转速为300r/min时,有利于溶氧和传质,400r/min时,由于剪切力过大,导致菌丝断裂,ε-PL产量下降。通过控制发酵中后期pH4.0和搅拌转速300r/min的pH反馈控制自动流加补料培养,可获得最大的ε-PL产量7.36g/L,产率0.072g/g,产量较优化前提高近10倍,产率提高2倍。Optimization of the ε-PL fermentation conditions in 50 L fermentor, the effects of pH and stirrer speed on the batch fermentation and fed-batch fermentation of ε-PL production were studied. The results showed that the growth of cells was promoted when pH remained at 5.0, but the synthesis of ε-PL was inhibited, even the accumulated ε-PL was depolymerized, while high ε-PL accumulation was achieved at pH 4. 0. Under the optimal condition, In late stage, controlling pH4.0 by glucose auto-feeding and stirrer speed 350r/min, the production of ε-PL was 7. 36g/L, the yield to glucose was 0. 072g/g, this control and feeding strategy enhanced the production of ε-PL 11 times.

关 键 词：Ε-聚赖氨酸 白色链霉菌 发酵 补料 PH控制 

分 类 号：TS202.3[轻工技术与工程—食品科学]