4种富硒植物预防MNNG诱发大鼠胃癌效果研究——不同源硒抑制大鼠胃窦黏膜上皮细胞异倍体发生率对比研究  

Preventive effects of 4 Se-enriched plants on rat stomach cancer induced by MNNG——1.Inhibitary effects of different selenium resources on rat aneuploid cell incidence in mucosal epithelium of gastric antrum

作　　者：杨文婕[1] 李卫东[1] 陈竞[1] 陈晓滨[1] 曲宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《卫生研究》2007年第5期612-614,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30170800)

摘　　要：目的筛选具有高效低毒特征的高健康价值硒。方法选择3种代表性富硒植物,以预防胃癌效果较好的富硒大蒜为生物硒对照材料,以亚硒酸钠为合成硒对照化合物,应用N-甲基-N′-硝基-N-亚硝基胍(MNNG)诱发大鼠胃窦黏膜上皮细胞异倍体发生(IAC)模型,调查比较5种富硒材料防癌效果。结果150μg/kgbw(以Se计,以下同)富硒大蒜组和富硒青花菜组大鼠IAC分别为25%和30%,两组间没有显著性差异;300μg/kgbw富硒大蒜组、富硒绿羽组和富硒红羽组大鼠IAC依次为22%、30%和50%,三组间没有显著性差异。150μg/kgbw亚硒酸钠组大鼠全部死亡,无法比较富硒植物与合成硒化合物亚硒酸钠的抑癌效果,但表明富硒植物毒性远小于亚硒酸钠。结论初步证实三种新选择的富硒植物具有高效低毒特征。Objective To obtain new Se resources with high healthy value （both high activity and low toxicity）, the preventive efficacies of three Se-enriched higher plants on stomach cancer were compared with selenite and Se-enriched garlic. Methods Ninety weanling male Wistar rats were fed the basal diet for a week, divided equally into nine groups, control, MNNG,Se 75 and 150μg/kg bw of selenite, Se 150 and 300μg/kg bw of Se-enriched garlic, Se 150μg/kg bw of Se-enriched broccoli, Se 300μg/kg bw of Se-enriched red kales and green kales group. Rats in MNNG and Se supplementation groups were daily given 15mg/kg bw of MNNG （solved in 1 ml distilled water）and the rats of control group were given lml distilled water by gavage for ten days. Meanwhile, the rats of the control and MNNG group were daily given 1 ml distilled water and the rats of other groups were given 1 ml water suspension of Se-enriched garlic, red kavas, green kavas, broccoli or 1 ml solution of sodium selenite by gavage for seventeen weeks. All rats were freely fed the basal diet and water during the period of the experiment. At the end of 18th week, the rats were sacrificed, the incidence of aneuploid cells （IAC） in mucosal epithelium of the gastric antrum was detected, and the IAC data were analyzed by SPSS 12.0.Results The results showed that the IACs were 25% and 30%, respectively, in the Se 150μg/kg bw of Seenriched garlic and -broccoli group, and were in turn 22% , 50% and 30% in the 300ttg/kg bw of Se-enriched garlic, -red kale and -green kale. No significant differences of IACs were found in the same level of Se supplementation groups by Seenriched plants. Conclusion The data showed that Se-enriched broccoli, red kale and green kale had high activities similar to Se-enriched garlic in stomach cancer prevention and lower toxicity than selenite.

关 键 词：富硒植物 胃癌预防 

分 类 号：Q581[生物学—生物化学] R151.2[医药卫生—营养与食品卫生学]