17beta-estradiol stimulates proliferation of spermatogonia in experimental cryptorchid mice  

作　　者：En-Zhong Li De-Xue Li Shi-Qing Zhang Chang-Yong Wang Xue-Ming Zhang Jing-Yan Lu Cui-Mi Duan Xiang-Zhong Yang Li-Xin Feng 

机构地区：[1]Beijing Institute of Basic Medical Sciences, Beijing 100850, China [2]Department of Agriculture and Forestry, Huanghuai University, Zhumadian 463000, China [3]College of Animal Sciences and Veterinary Medicine.Jilin llniversitv. Changchun 170002. China [4]Department of Animal Science and Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut 06269-4243, USA

出　　处：《Asian Journal of Andrology》2007年第5期659-667,共9页亚洲男性学杂志（英文版）

基　　金：Acknowledgment This work was supported by the National Natural Science Foundation of China （No. 30200195）. We thank Dr Hai-Bin Wang for taking photographs and Dr Su-Hui Wu （Henan Normal University, China） for statistical analysis. We thank the faculty of Huanghuai University for supporting Dr En-Zhong Li.

摘　　要：Aim： To investigate whether estrogen stimulates the proliferation of spermatogonia or induces spermatogenesis in cryptorchid mice. Methods： Mice were surgically rendered cryptorchid, then treated with different doses of 17β- estradiol （E2） s.c. once a day. Mice were killed at sexual maturity （45 days of age）, and histological analysis and inununofluorescence were performed. Serum follicle stimulating hormone （FSH）, estradiol, testosterone and luteinizing hormone （LH） were measured. Results： Low doses of E2 had no notable effect on spermatogonia, but at higher doses, E2 stimulated the proliferation of spermatogonia. Conclusion： E2 has a dose-related mitogenic effect on spermatogonia.Aim： To investigate whether estrogen stimulates the proliferation of spermatogonia or induces spermatogenesis in cryptorchid mice. Methods： Mice were surgically rendered cryptorchid, then treated with different doses of 17β- estradiol （E2） s.c. once a day. Mice were killed at sexual maturity （45 days of age）, and histological analysis and inununofluorescence were performed. Serum follicle stimulating hormone （FSH）, estradiol, testosterone and luteinizing hormone （LH） were measured. Results： Low doses of E2 had no notable effect on spermatogonia, but at higher doses, E2 stimulated the proliferation of spermatogonia. Conclusion： E2 has a dose-related mitogenic effect on spermatogonia.

关 键 词：17Β-ESTRADIOL cryptorchid mice PROLIFERATION SPERMATOGONIA 

分 类 号：R-332[医药卫生]